EFFECTS OF PRETREATMENT METHODS ON THE LIGNOCELLULOSIC COMPOSITION OF SALACCA MIDRIBS
DOI:
https://doi.org/10.26877/bioma.v15i1.3473Keywords:
Cellulose; Hemicellulose; Lignin; Pretreatment; Salacca midribAbstract
This study aims to determine the effect of pretreatment on hemicellulose, cellulose, and lignin content of Salacca midrib. Unlike previous studies, this study was designed to evaluate the effects of single-, double-, and triple-stage pretreatments on the sallaca midrib. The study was also designed to evaluate the differences in the physical and biological pretreatment methods. The pretreatments applied consist of physical, chemical, biological, physicochemical, chemical-biological, physical-biological, and physicochemical-biological pretreatments. Physical pretreatment is carried out in an autoclave at 121 °C, chemical pretreatment using Sodium Hydroxide (NaOH) 4%, and biological pretreatment using Debaryomyces hansenii. Based on the ANOVA test, the various pretreatment methods have a significant effect on lignocellulose content. The highest hemicellulose content was the chemical pretreatment at 34.13%, the cellulose content was the chemical pretreatment at 40.85%, and the highest lignin reduction was the physical-chemical-biological at 27.15%
References
Albersheim, P., Darvill, A., Roberts, K., Sederoff, R., & Staehelin, A. (2011). Plant cell walls: from chemistry to biology. Garland Science.
Abolore, R. S., Jaiswal, S., & Jaiswal., A. K. (2025). A comprehensive review on sustainable lignin extraction techniques, modifications, and emerging applications. Industrial Crops and Products, 235, 1-19. DOI:10.1016/j.indcrop.2025.121696
Atidhira, Y., Noviansyah, A., & Taufany, F. (2017). Pengembangan metode pretreatment melalui proses fisik dan kimia untuk optimasi produksi biogas dari Eceng Gondok (Eichhornia crassipes) sebagai alternatif energi listrik ─ Biogas. Teknik ITS, 6(2), 247-251.
Chen, J., Ma, X., Liang, M., Guo, Z., Cai, Y., Zhu, W., Wang, S., Xu, J., & Ying, H. (2024). Physical-chemical–biological pretreatment for biomass degradation and industrial applications: A Review. Waste, 2, 451–473. https://doi.org/10.3390/waste2040024
Devi, D., Astutik, D., Cahyanto, M.N., & Djaafar, T. F. (2019). Kandungan lignin, hemiselulosa dan selulosa pelepah salak pada perlakuan awal secara fisik, kimia, dan biologi. Ilmiah Rekayasa Pertanian dan Biosistem, 7(2), 273-282. DOI:10.29303/jrpb.v7i2.148
Fan, J., Lu, Y., An, N., Zhu, W., Li, M., Gao, M., Wang, X., Wu, C., and Wang, Y. (2025). Pretreatment technologies for lignocellulosic biomass: research progress, mechanisms, and prospects. BioResources, 20(2), 4897-4924. https://doi.org/10.15376/biores.20.2.Fan.
Harahap, M. F., Lubis, R. A., Syawaluddin., Silitonga, Y. W., & Harahap, I. S. (2020). The quality of salacca tree midrib latex flour as a thickening agent. Physics: Conference Series, 1477, 1-6. https://doi.org/10.1088/1742-6596/1477/7/072008.
Harmsen, P. F. H., Huijgen, W. J. J., Bermúdez López, L. M., & Bakker, R. R. (2010). Literature review of physical and chemical pretreatment processes for lignocellulosic biomass (ECN-E--10-013). Energy Research Centre of the Netherlands, 1-49.
Hernandez, S. C., Suzuki, S., Wada, N., & Takahashi, K. (2024). Ionic liquid pretreatment of lignocellulose for complete hemicellulose removal to produce high-purity cellulose mixed esters. Industrial Crops and Products, 218. DOI:10.1016/j.indcrop.2024.118909
Jacquet, N., Djakovitch, F., & Dumeignil, F. (2015). Oxidative depolymerization of lignins for producing aromatics in aqueous medium under mild conditions. Catalysis Today, 257, 161–174.
Hafizuddin, M. S., Lee, C. L., Chin, K. L., H’ng, P. S., Khoo, P. S., & Rashid, U. (2021). Fabrication of highly microporous structure activated carbon via surface modification with sodium hydroxide. Polymers, 13(22), 1-16. https://doi.org/10.3390/polym13223954
Lestari, M. D., Sudarmin, S., & Harjono, H. (2018). Ekstraksi selulosa dari limbah pengolahan agar menggunakan larutan NaOH sebagai precursor bioetanol. Indonesian Chemical Science, 7(3), 236-241. https://doi.org/10.15294/CHEMINED.V1212.69048.
Lismeri, L., Sari, R. M., & Sari, N. M. (2018). Efektivitas pretreatment limbah ampas tahu menggunakan NaOH terhadap produksi bioetanol. Teknik Kimia USU, 4(1), 1–6. https://doi.org/10.33536/jcpe.v4i1.319.
Ming, X., Wang, Y., & Sui, Y. (2020). Pretreatment of the antagonistic yeast, Debaryomyces hansenii, with mannitol and sorbitol improves stress tolerance and biocontrol efficacy. Frontiers in Microbiology, 11(601), 1-10. https://doi.org/10.3389.fmicb.2020.00601.
Naufala, W. A dan E. S. Pandebesie. (2016). Hidrolisis eceng gondok dan sekam padi untuk menghasilkan gula reduksi sebagai tahap awal produksi bioetanol. Teknik ITS. 4(2), 109-113. https://www.neliti.com/publications/193425/hidrolisis-eceng-gondok-dan-sekam-padi-untuk-menghasilkan-gula-reduksi-sebagai-t
Pramasari, D. A., Oktaviani, M., Thontowi, A., Purnawan, A., Ermawar, R. A., Sondari, D., Ningrum, R. S., Laksana, R. P. E., Lianawati, A., Fahrezi, M. Z. M., Salsabila, Q., & Hermiati, E. 2023. The use of hemicellulose acid hydrolysate for hydrolysis of sugarcane trash and its fermentation for producing xylitol. Industrial Crops and Products, 193(23):116-163 DOI:10.1016/j.indcrop.2022.116163
Rafidah, J., Mohd-Sahaid, K., Norliza, A.R., Aidil, A.H., & Mohd-Farid, A. (2020). Effect of sodium hydroxide pretreatment on chemical composition of treated acacia mangium using response surface methodology. Tropical Forest Science, 32(4), 391-401. DOI:10.26525/jtfs2020.32.4.391
Rahayu, S., Supiyani, A., Darmansyah, R., & Amalia, R. 2022. Pretreatment minyak jelantah dengan karbon aktif ampas tebu menurunkan resiko hepatotoksisitas tikus (Spraque dawley), Bioma: Biologi UNJ Press, 18(2), 83-90. https://doi.org/10.21009/Bioma18(2).5
Ruli, F. F. (2025). Khamir penghasil lipid yang memanfaatkan biomassa lignoselulosa untuk produksi biodiesel. Bioma: Biologi Makasar, 10(2), 20-30.
Silaban, R., & Harahap, A. U. (2021). Produksi dan komposisi nutrisi limbah pelepah tanaman salak yang difermentasi dengan kapang pelapuk putih (Phanerochaete chrysosporium). Livestock and Animal Health, 4(2), 15-20. DOI:10.32530/jlah.v4i1.317
Triyastiti, L., & Krisdiyanto, D. (2017). Isolasi nanoselulosa dari pelepah pohon salak sebagai filler pada film berbasis Polovinil Alkohol (PVA). Materials Chemistry, 1(1), 39-45. https://ejournal.uin-suka.ac.id/saintek/IJMC/article/view/1265/1090
Wang, X., Meng, Y., Zhang, J., & Li, Y. (2020). Production and characterization of extracellular xylanolytic enzyme by Debaryomyces hansenii strains. Industrial Microbiology and Biotechnology, 47(8), 615-627.
Widyorini, R., Soraya, A., & Sari, R. M. (2015). Kandungan lignin, hemiselulosa dan selulosa pelepah salak pada perlakuan awal secara kimia, fisik dan biologi. Rekayasa Pertanian dan Biosistem, Universitas Mataram, 3(2), 148–153. DOI:10.29303/jrpb.v7i2.148
Zhang, J., Liu, J., Kou, L., Zhang, X., & Tan, T. (2019). Bioethanol production from cellulose obtained from the catalytic hydro-deoxygenation (lignin-first refined to aviation fuel) of apple wood. Fuel, 250 (2019), 245-253. DOI:10.1016/j.fuel.2019.03.020





